Cancer is the leading causes of death worldwide. Development of new effective drugs to combat the cancers is still needed. Because of similar genetic and histopathologic features between zebrafish and human, zebrafish has been developed as a promising model for cancer research. The strategic position of zebrafish in anti-cancer drug discovery is to bridges between cell-based and mammalian models in pre-clinical drug screening along the effective drug development pipeline.
To assess drug effect, forty eight to fifty six hours post-fertilization zebrafish embryos will be anesthetized with tricaine. The suspend cancer cells will be stained with DiI for 30 minutes at 37oC, and then about 100-300 DiI-stained cells will be injected into the yolk sac of embryos and for drug treatment. Afterwards, the photographs of embryos will be taken and analyzed.
2. Service items：Fish line maintenance, zebrafish breeding, microinjection, drug testing
|Service||On campus||Cooperation||Off campus|
|Wild type (adult)||150/pair||180/pair||225/pair|
|Wild type (embryo)||150/100 embryos||180/100 embryos||225/100 embryos|
|Transgenic fish (adult)||500||600||750|
|Paramecium||300/600 ml||360/600 ml||450/600 ml|
|Fish line screening||2400/day||2880/day||3600/day|
|Breeding & maintain for one month||3000/60 embryos||3600/60 embryos||4500/60 embryos|
|Maintenance (one-month-old to three-month-old)||200/tank||240/tank||300/tank|
4. Related Forms：
研發處> 收費平台> 傳染病與癌症研究中心> 人類癌症斑馬魚模式藥物篩選平台
Contact person：Wang-ta Liu